Achieving comprehensive analysis and characterization of extracellular vesicles
Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.
Accurately characterizing extracellular vesicles (EVs) can be challenging, even in highly purified cell culture samples. Interference from lipoproteins, cell debris, and protein aggregates can pose challenges. In addition, complex biofluids, limited sample volumes, and rare EV subpopulations can all add further complexity.
Leprechaun provides a solution by isolating EVs on its Luni consumable before measuring particle size and concentration, and analyzing EV phenotype for up to 4 surface or cargo markers simultaneously, from <25 µL of sample. It can be applied to a range of materials without the need for sample purification, while providing sensitivity down to 5×10^5 particles/mL, single particle analysis, and the ability to size EVs as small as 35 nm.
In this webinar, you’ll learn how Leprechaun combines single particle interferometry and immunofluorescence imaging to provide comprehensive analysis of any EV sample. Leprechaun can also check loading efficiency of EV packaged cargo, ID whether a protein is soluble or EV-associated, and provide a complete breakdown of all EV subpopulations.
- Learn how to perform single particle analysis of EVs and exosomes in crude and pure samples
- Discover how to obtain concentration, size, and phenotype information for any subpopulation of EVs
- Discover how to analyze protein and nucleic acid EV cargo