Embrace simple nucleic acid desalting and quantification with Unagi and Lunatic
The Problem:
Desalting and quantifying nucleic acids is essential for ensuring accuracy and efficiency for downstream assays, but the process can be challenging. Manual methods of desalting are time-consuming and costly. Fluorescence-based methods of quantification require standards, dyes and dilutions while the accuracy of traditional UV absorbance is affected by impurities or varying sequences.
The Solution:
Unagi makes desalting a breeze. Simply load up to 8 samples of 8 mL and step away while Unagi removes solvents like ACN, salts and small molecules. End up with a purified product in any buffer, that can even be concentrated up to 16-fold.
Lunatic guarantees accurate UV/Vis quantification from just 2 μL of sample – dye and dilution free!
The Proof:
Tune in to see how simple it is to set up a desalting concentration run on Unagi and how Lunatic quickly and accurately quantifies nucleic acids with different sequences.
Learning Objectives
- Discover how buffer exchange can make desalting plug-and-play
- How to use UV/Vis to quantify DNA and RNA, dye-free
- Conclude that desalting, concentrating and quantifying nucleic acids can be done with minimal hands-on time