Accelerated Label-free Kinetics Screening in Early Single Domain Antibody Discovery

A Comparative Analysis with Phage Display Samples from Periplasma and Comparison with Orthogonal Technologies

This study investigates the efficacy of rapid and high-throughput label-free kinetics screening in the early stages of antibody discovery, particularly focusing on phage display samples obtained from periplasma. The utilization of BLI (bio-layer-interferometry) during kinetics screening allows for swift analysis and evaluation of antibody performance, offering valuable insights into binding kinetics. The results obtained from this method are compared with those derived from orthogonal technologies, providing a comprehensive assessment of antibody characteristics. Through this comparative analysis, the suitability and reliability of label-free biosensor technology for kinetics screening are assessed in the context of antibody discovery, offering valuable implications for optimizing screening methodologies in early-stage drug development.